Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Composite

Part:BBa_K1404001:Design

Designed by: Alexandre Duprey   Group: iGEM14_INSA-Lyon   (2014-09-23)

Ptac-NiCoTB-rcn-csgBAEFG, turns E. coli into a functional Ni and Co biofilter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1361
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 857
    Illegal AgeI site found at 1056
    Illegal AgeI site found at 3422
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part combines the enhanced metal accumulation capacities from K1404002 and the improved adherence from K540000 .

Source

K1404002 and K540000 were cloned by 3A assembly.

References

[1] Duprey A, Chansavang V, Fremion F, Gonthier C, Louis Y, Lejeune P, Springer F, Desjardin V, Rodrigue A, Dorel C: “NiCo Buster”: engineering E. coli for fast and efficient capture of cobalt and nickel. J Biol Eng 2014, 8:19.